Culture media comparative assessment of common fig (Ficus carica L.) and carryover effect

Production of common fig in jordan has dropped more than 20-folds during the last five decades,due to several challenges,including biotic and abiotic stresses. the present study was conducted to asses a new protocol of shoot and callus development from fig apical buds. explants of three local genotypes (khdari,mwazi and zraki) were collected and grown in three culture establishment media: murashige and skoog (ms),olive medium (om) and woody plant medium (wpm). two months later,shoot growth and callus development were measured,followed by transplanting into subculturing medium in test tubes with ms media. after additional two months,the same parameters were measured again. the results of the culture establishment showed that the highest shoot growth was obtained with om and the highest callus development was obtained with wpm. on the other hand,the subculturing in ms medium showed prominent carryover effect of the first inoculation media,where the highest shoot growth was obtained in cultures transplanted from om and the highest callus development was obtained in cultures transplanted from wpm. the present study delivers an improved protocol for establishment of common fig by tissue culture.