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First record of leech Dina punctata (Annelida: Erpobdellidae) from Lesser Zab River in northern Iraq: Morphological and molecular investigation
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نویسنده
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bilal s.j. ,ali l.a. ,abdullah l.y. ,khailany r.a. ,dhahir s.f. ,abdullah s.m.a.
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منبع
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jordan journal of biological sciences - 2017 - دوره : 10 - شماره : 2 - صفحه:69 -72
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چکیده
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A total of 17 specimens of the leech dina punctata were collected from lesser zab river in zirandul region near qashqoli village during the period from july to october 2015. specimens were examined either live using dissecting microscope,or after being fixed in 5% formaldehyde. specimens used for molecular analysis of genomic dna were fixed and preserved in absolute ethanol. following dna extraction,region of 28s rdna was amplified by polymerase chain reaction (pcr),and the nucleotides order was determined using genetic analyzer. morphological and morphometric futures as well as molecular analysis showed that the collected specimens belonged to d. punctata. this is the first record for this species in iraq. © 2017 jordan journal of biological sciences.
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کلیدواژه
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28S rDNA marker; Dina punctata; DNA sequencing
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آدرس
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department of fish resource and aquatic animal,college of agriculture,salahaddin university,erbil,iraq,department of biology,faculty of eduaction,university of ishik,erbil, Iraq, department of biology,college of education,salahaddin university,erbil, Iraq, scienctific research center,salahaddin university,erbil, Iraq, department of biology,college of science,salahaddin university,erbil,iraq,department of medical biology,faculty of medicine,gaziantep university,gaziantep, Turkey, department of biology,college of education,salahaddin university,erbil, Iraq, department of fish resource and aquatic animal,college of agriculture,salahaddin university,erbil, Iraq
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Authors
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