Molecular cloning and in Silico analysis of rps7 gene from the Lactuca sativa

The rps7 genes encode 30s ribosomal protein s7 that bind directly to the parts of the 3'end of 16s ribosomal rna. the rps7 gene is one of the most important plastidial genes that have not been studied so far in lettuce (lactuca sativa). the molecular analysis of rps7 gene provides an opportunity to make phylogenetic studies and a conserved homologous recombination site for designing chloroplastic expression vectors with high-efficiency lettuce cultivars and its related species. here,we have cloned plastid rps7 gene from lettuce. the full length of lettuce rps7 gene is 2579 bp,composed of the 289 bp promoter region,an exon 468 bp long encoding 155 amino acids. the rps7 gene contains a conserved domain (1-155). the promoter region analysis showed that the cloned fragment contains motifs,like tata box,caat-box,mbs,aagaa-motif and ga-motif. the alignment analysis rps7 showed the high homology with identity of 96% compared with asteraceae family at the nucleotide level,while the amino acid sequence of the gene showed a high similarity value of 98.1-90% compared with hairy sunflower,wormwood,ragwort and soybean. the phylogenetic analysis result shows the rps7 gene from dicots is close to rps7 gene from lettuce varieties. © 2017 jordan journal of biological sciences.