HPLC-DAD fingerprinting analysis,antioxidant activity of phenolic extracts from Blighia sapida Bark and its inhibition of cholinergic enzymes linked to Alzheimer's disease

In west africa,the stem of blighia sapida k.d. koenig are commonly used as remedy against a variety of diseases,including neurodegenerative diseases without scientific basis. the present study characterizes the phenolic constituents,assessed the cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) and evaluated the antioxidant properties of phenolic extracts from b. sapida k.d. koenig. total phenol and flavonoids content was evaluated as well as antioxidants as illustrated by fe2+ chelation,2,2-diphenyl-1-picrylhydrazyl (dpph·) radical scavenging ability and 2,2-azino-bis-(3-ethylbenthiazoline-6- sulphonic acid (abts·) radical scavenging ability spectophotometrically. the ability of the extract to inhibit the activities of acetylcholinesterase and butyrylcholinesterase was also evaluated. the extract was found to be rich in phenolic acid (gallic acid,ellagic acid) and flavonoids (quercetrin and luteolin). the results show that the phenolic extracts had dpph radical scavenging abilities (ic50 = 90.71 μg/ml),abts· radical scavenging ability (ic50 = 85.47 μg/ml),iron chelation (ic50 = 136.61 μg/ml) and reducing power (fe3+- fe2+) (400.08 aae mg/100g). extracts of b. sapida inhibited acetylcholinesterase (ache) (ic50 = 125.56 μg/ml) and butyrylcholinesterase (bche) (ic50 = 230.63 μg/ml) activities in a concentration dependent manner (20-100 μg/ml). hence,one probable means through which the stem bark execute their anti-alzheimer's disease activity might be by inhibiting cholinesterase activities in addition to thwarting oxidative-stress-induced neurodegeneration. © 2017 jordan journal of biological sciences.