Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by lactobacillus sp. A-2 metabolites

Objective: to study the effect of lactobacillus sp. a-2 metabolites on viability of cal-27 cells and apoptosis in cal-27 cells. methods: lactobacillus sp. a-2 metabolites 1 and 2 (lm1 and lm2) were obtained by culturing lactobacillus sp. a-2 in reconstituted whey medium and whey-inulin medium; the cultured cal-27 cells were treated with different concentrations of lm1 and lm2 (0,3,6,12,24,48 mg/ml) and assayed by methyl thiazolyltetrazolium (mtt) method; morphological changes of apoptotic cell were observed method (fcm) and agarose gel electrophoresis were used to detect the apoptosis of cal- 27 cells treated with lm1 and lm2. results: the different concentrations of lm1 and lm2 could restrain the growth of cal-27 cells,and in a dose-dependent manner; the apoptosis of cal-27 cells was obviously induced and was time-dependent. conclusions: viability of cal-27 cells was inhibited by lactobacillus sp. a-2 metabolites; lactobacillus sp. a-2 metabolites could induce cal-27 cells apoptosis; study on the bioactive compounds in the lactobacillus sp. a-2 metabolites and their molecular mechanism is in progress.