Bacteriological analysis of necrotic pulp and fstulae in primary teeth

Objectives: primary teeth work as guides for the eruption of permanent dentition,contribute for the development of the jaws,chewing process,preparing food for digestion,and nutrient assimilation. treatment of pulp necrosis in primary teeth is complex due to anatomical and physiological characteristics and high number of bacterial species present in endodontic infections. the bacterial presence alone or in association in necrotic pulp and fstula samples from primary teeth of boys and girls was evaluated. material and methods: necrotic pulp (103) and fstula (7) samples from deciduous teeth with deep caries of 110 children were evaluated. bacterial morphotypes and species from all clinical samples were determined. results: a predominance of gram-positive cocci (81.8%) and gram-negative coccobacilli (49.1%) was observed. in 88 out of 103 pulp samples,a high prevalence of enterococcus spp. (50%),porphyromonas gingivalis (49%),fusobacterium nucleatum (25%) and prevotella nigrescens (11.4%) was observed. porphyromonas gingivalis was detected in three out of seven fstula samples,enterococcus spp. in two out of seven samples,and f. nucleatum,p. nigrescens and d. pneumosintes in one out of seven samples. conclusions: our results show that enterococcus spp. and p. gingivalis were prevalent in necrotic pulp from deciduous teeth in boys from 2 to 5 years old,and that care of the oral cavity of children up to fve years of age is important.