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Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway
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نویسنده
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yi j. ,li m.l. ,yang y. ,zheng w. ,li y. ,zhao z.
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منبع
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journal of applied oral science - 2015 - دوره : 23 - شماره : 6 - صفحه:549 -554
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چکیده
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Objective: this study aimed to investigate the potential role of camk ii pathway in the compression-regulated opg expression in periodontal ligament cells (pdlcs). material and methods: the pdl tissue model was developed by 3-d culturing human pdlcs in a thin sheet of poly lactic-co-glycolic acid (plga) scaffolds,which was subjected to static compression of 25 g/cm2 for 3,6 and 12 h,with or without treatment of kn-93. after that,the expression of opg,rankl and nfatc2 was investigated through real-time pcr and western blot analysis. results: after static compression,the nfatc2 and rankl expression was significantly up-regulated,while partially suppressed by kn-93 for 6 and 12 h respectively. the opg expression was significantly down-regulated by compression in 3 h,started to elevate in 6 h,and significantly up-regulated in 12 h. the up-regulation after 12 h was significantly suppressed by kn-93. conclusions: long-term static compression increases opg expression in pdlcs,at least partially,via the camk ii pathway. © 2015,faculdade de odontologia de bauru. all rights reserved.
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کلیدواژه
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Calcium-calmodulin-dependent protein kinases; Mechanotransduction; Osteogenesis; Periodontal ligament
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آدرس
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sichuan university,west china school and hospital of stomatology,state key laboratory of oral diseases,department of orthodontics,chengdu, China, sichuan university,west china school and hospital of stomatology,state key laboratory of oral diseases,department of orthodontics,chengdu, China, sichuan university,west china school and hospital of stomatology,state key laboratory of oral diseases,department of orthodontics,chengdu, China, sichuan university,west china school and hospital of stomatology,state key laboratory of oral diseases,department of oral and maxillofacial surgery,chengdu, China, sichuan university,west china school and hospital of stomatology,state key laboratory of oral diseases,department of orthodontics,chengdu, China, sichuan university,west china school and hospital of stomatology,state key laboratory of oral diseases,department of orthodontics,chengdu, China
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Authors
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