Human plasma fibronectin promotes proliferation and differentiation of odontoblast

Objective to assess the effect of fibronectin (fn) and porcine type i collagen (pcol) on odontoblast-like cells in vitro. material and methods rat odontoblast-like cells (mdpc-23 cells) were inoculated and cultured on fn-coated or type i collagen-coated substrates. proliferation assay,alkaline phosphatase activity (alp activity),mrna expression of hard tissue-forming markers,and alizarin red staining were investigated over a period of 10 days. results cells maintained a high proliferation activity on fn and pcol even at a low seeding concentration (0.5×104/ml) as demonstrated by cck-8 assay. the proliferation activity of cells on fn increases in a concentration-dependent manner while it reached a plateau after 10 µg/ml. cells adopted long,thin and spindle shape on fn(10-50) and pcol. parallel actin filaments were observed in mdpc-23 cells cultured on fn and pcol. alp activity was markedly up-regulated on fn and pcol-coated surfaces. importantly,gene expression of bsp (fn10: 2.44±0.32; fn20: 3.05±0.01; fn30: 2.90±0.21; fn40: 2.74±0.30; fn50: 2.64±0.12; pcol: 2.20±0.03) and ocn (fn10: 2.52±0.23; fn20: 2.28±0.24; fn30: 2.34±0.21; fn40: 2.34±0.25; fn50: 2.20±0.22; pcol: 1.56±0.16) was significantly enhanced on fn and pcol substrates as compared with control; moreover,expression of integrin beta 1 (itgb1),an ubiquitous cell surface receptor was augmented in fn(10-50) and pcol groups simultaneously. in accordance with the alp activity and gene expression data,calcific deposition in cells grown on fn(10-50) and pcol was observed as well. conclusion despite the limitation of this study,the findings indicate that a surface coating of fn enhances the proliferation,differentiation and mineralization of odontoblast-like cells by activation of integrin beta 1 (itg b1). the promoting effects of fn on mdpc-23 cells were achieved at a comparatively lower coating concentration than type i collagen (300 µg/ml). specifically,it is suggested that the optimum coating concentration of fn to be 10 µg/ml. © 2017,faculdade de odontologia de bauru. all rights reserved.