Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride

Objectives: methylcellulose (mc) is a chemical compound derived from cellulose. mta mixed with mc reduces setting time and increases plasticity. this study assessed the influence of mc as an anti-washout ingredient and cacl2 as a setting time accelerator on the physical and biological properties of mta. material and methods: test materials were divided into 3 groups; group l(control): distilled water; group 2: 1% mc/cacl2; group 3: 2% mc/ cacl2,compressive strength,ph,flowability and cell viability were tested. the gene expression of bone sialoprotein (bsp) was detected by rt-pcr and realtime pcr. the expression of alkaline phosphatase (alp) and mineralization behavior were evaluated using an alp staining and an alizarin red staining. results: compressive strength,ph,and cell viability of mta mixed with mc/ cacl,were not significantly different compared to the control group. the flowability of mta with mc/cacl has decreased significantly when compared to the control (p<.05). the mrna level of bsp has increased significantly in mta with mc/cacl2 compared to the control (p<.05). this study revealed higher expression of alp and mineralization in cells exposed to mta mixed with water and mta mixed with mc/cacl2 compared to the control (p<.05). conclusions: mc decreased the flowability of mta and did not interrupt the physical and biological effect of mta. it suggests that these cements may be useful as a root-end filling material. © 2017,faculdade de odontologia de bauru. all rights reserved.