Effects of triethylene glycol dimethacrylate (Tegdma) on the odontoclastic differentiation ability of human dental pulp cells

Objectives: the primary purpose of this study was to examine the effects of triethylene glycol dimethacrylate (tegdma) on odontoclastic differentiation in the dental pulp tissue. material and methods: the effects of different tegdma dosages on the odontoclastic differentiation capability of dental pulp cells were analyzed in vitro using the following methodologies: i) flow cytometry and tartrate-resistant acid phosphatase (trap) staining; ii) apoptotic effects using annexin v staining; iii) mrna expression of osteoprotegerin (opg) and receptor activator of nuclear factor (nf)-kb ligand (rankl) genes by quantitative real-time pcr (qrt-pcr); and iv) opg and rankl protein expression by enzyme-linked immunosorbent assay (elisa). results: tegdma caused relatively less odontoclastic differentiation in comparison with the control group; however,odontoclastic differentiation augmented with increasing doses of tegdma (p<0.05). the mrna and protein expression of opg was lower in tegdma treated pulp cells than in the control group (p<0.05). while the mrna expression of rankl remained unchanged compared to the control group (p>0.05),its protein expression was higher than the control group (p<0.05). in addition,tegdma increased the apoptosis of dental pulp cells dose dependently. conclusions: tegdma reduced the odontoclastic differentiation ability of human dental pulp cells. however,odontoclastic differentiation ratios increased proportionally with the increasing dose of tegdma. © 2017,faculdade de odontologia de bauru. all rights reserved.