Roles of fibrin deposition and protease activated receptor-1 in renal cytokine/chemokine production and inflammatory cell infiltration in rats of different ages

The present study aimed to investigate the roles of fibrin deposition and protease activated receptor-1 (par-1) in renal cytokine/chemokine production and inflammatory cell infiltration in rats of different ages. acute inflammation was induced by lipopolysaccharide (lps) in rats which were then treated with tranexamic acid(ta),ta+urokinase (uk) or ta+low-molecular- weight heparin (hp). fibrin deposition,inflammatory cells and expressions of par-1,monocyte chemoattractant protein-1 (mcp-1) and intercellular adhesion molecule 1 (icam-1) were detected. a reduction in fibrin deposition and par-1 expression in the lps+ta+hp group was associated with decreased infiltration of inflammatory cells and down-regulated expressions of mcp-1 and icam-1. in the lps+ta+uk group,the fibrin deposition,but not the par-1 expression,was reduced,however,the infiltration of inflammatory cells decreased and the expressions of mcp-1 and icam-1 down-regulated. there were significant differences in the fibrin deposition,infiltration of inflammatory cells and expression of par-1,mcp-1 and icam-1 between young and old rats undergoing the same treatment. these findings demonstrated that fibrin deposition plays more important roles than par-1 dose in cytokine/chemokine production and inflammatory cell infiltration in vivo,and ageing may deteriorate the fibrin deposition-induced production of cytokines/chemokines and infiltration of inflammatory cells. © journal of applied biomedicine.