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BMP-2 modulates expression of other growth factors in a rat fracture healing model
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نویسنده
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cottrell j.a. ,keane o. ,lin s.s. ,o'connor j.p.
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منبع
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journal of applied biomedicine - 2014 - دوره : 12 - شماره : 3 - صفحه:127 -135
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چکیده
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Recombinant human bone morphogenetic protein-2 (rhbmp-2) is an osteo-inductive reagent that can be used therapeutically to promote spinal arthrodesis and fracture healing. despite its clinical use,the effect of rhbmp-2 on expression of endogenous osteogenic growth factors is poorly described. the goal of this study was to determine if local rhbmp-2 treatment modulates expression of pro-osteogenic growth factors during fracture healing. rat femur fractures were wrapped with control or rhbmp-2 collagen soaked sponge. radiographic observations demon- strated that rhbmp-2 treatment enhanced fracture callus formation and decreased bridging time. expression of fracture site growth factor mrnas was determined by quantitative poly- merase chain reaction. the gene expression data demonstrated that rhbmp-2 treatment significantly increased mrna levels of bmp-2 at day 4,bmp-4 at days 2,4,7,and 10,bmp-7 at day 2,and tnf-α at day 7. treatment with rhbmp-2 was shown to significantly reduce mrna levelsof bmp-2 onday2 and10,tnf-α on day 14,and bmp-6 ondays 2,10,14,and 21.tgfβ-1 was also significantly reduced on days 2 and 21. in conclusion,treatment with rhbmp-2 appears to enhance expression of other osteogenic factors that could contribute to bone formation. © 2014 faculty of health and social studies,university of south bohemia in ceske budejovice. published by elsevier urban & partner sp. z o.o. all rights reserved.
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کلیدواژه
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Fracture healing; Growth factors; Rat RT-qPCR; Recombinant human bone morphogenetic protein-2
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آدرس
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department of biochemistry and molecular biology,rutgers university,new jersey medical school,msb e659,185 south orange avenue,newark,nj 07103,united states,department of biological sciences,seton hall university,school of arts and science,south orange,nj, United States, trinity college,university of dublin,dublin, Ireland, department of orthopaedics,rutgers university,new jersey medical school,newark,nj, United States, department of biochemistry and molecular biology,rutgers university,new jersey medical school,msb e659,185 south orange avenue,newark,nj 07103, United States
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Authors
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