Electromagnetic field (10 Hz,1 mT) protects mesenchymal stem cells from oxygen-glucose deprivation-induced cell death by reducing intracellular Ca2+ and reactive oxygen species

Protective effects of electromagnetic fields (emfs) against oxygen and glucose deprivation (ogd)-induced human mesenchymal stem cell (msc) death were studied. cell survival,intracellular calcium and ros/rns levels were measured after culturing mscs for 3 h under ogd with or without emf exposure. the survival rate of cells cultured under ogd condition was significantly reduced compared to control cells,while cells cultured in ogd with 10 hz/1 mt emf exposure had higher survival ratio than that in equivalent non-exposed cells. this protective effect of emf was not observed at different frequency/intensity combinations such as 10 hz/0.01 mt,10 hz/0.1 mt,50 hz/1 mt and 100 hz/1 mt. ros/rns levels of cells cultured under ogd conditions significantly increased compared to the control level while 10 hz/1 mt emf alleviated this effect. intracellular calcium levels in ogd group were higher than control while those in ogd plus 10 hz/1 mt emf group were significantly lower than ogd group. addition of ca2+ chelator promoted protective effects of emf against ogd-induced msc death. our results suggest that 10 hz/1 mt emf exposure protects mscs from ogd-induced cell death and the underlying mechanisms of the protection are reduction of intracellular levels of ca2+ and ros/rns. © 2016 faculty of health and social sciences,university of south bohemia in ceske budejovice