RGD peptide doped polypyrrole film as a biomimetic electrode coating for impedimetric sensing of cell proliferation and cytotoxicity

A novel and facile in vitro cell sensing system has been developed with one-step electropolymerization of the conducting polypyrrole(ppy) polymer using rgd peptide as the sole dopant on an indium tin oxide (ito) surface. the resulted rgd peptide-doped polypyrrole (ppy/rgd) composite film had a robust surface,in which ppy provided a biocompatible matrix for cell growth and a conducting interface for electrical detection,while the rgd peptide entrapped in the ppy matrix conferred the desired biomimetic properties. using the human lung cancer cell a549 as a model,this system can be used to monitor cell behaviors of proliferation and cytotoxicity. © 2017 faculty of health and social sciences,university of south bohemia in ceske budejovice