Molecular characterization of overproducing L-asparaginase recombinant Bacillus fusants

This work aimed to characterize recombinant strains,with a high production level of l-asparaginase,previously obtained by protoplast fusion between bacillus subtilis and bacillus cereus. the genetic relationship between fusants and their parents was determined using a simple pcrrestriction fragment length polymorphism (rflp) of 16s rdnas -based method,and by analyzing the specific l-asparaginase genes and protein patterns. our results indicate that all fusants have morphological characteristics and specific l-asparaginase genes similar to the bacillus cereus strain whereas protein pattern and 16s rdnas -rflp analysis show mixed characteristics from both parents. different genetic relationships were found between each fusant and its parental strain. these results confirm that protoplast fusion is an important technique in strain improvement. it was used to combine genes from different organisms for creating strains with desired properties.