In vitro plant regeneration from callus in semi dwarf cultivar of punica granatum L. 'Nana'

Leaf explants of a semi dwarf cultivar of pomegranate were placed on murashige and skoog [6] (ms) medium supplemented with various concentrations of 6-benzyl adenin (ba) and naphthalene acetic acid (naa) for callus induction. maximum callus induction was observed on a media containing 1 mg l-1 ba with 0 mg l-1 naa. however,the highest callus growth wasobtained on a medium containing 1 mg l-1 ba and 0.5 mg l-1 naa.. maximum shoot proliferation was observed when shoots were cultured on woody plant medium (wpm) supplemented with 2 mg l-1 ba. in this treatment,after 4 subcultures,85 shootswere produced from one original explant. among treatments used in rooting experiments,shoots cultured on wpm medium with 0-0.2 mg l-1 indol butyric acid (iba) had the maximum root percentage (100%). rooted plantlets were cultured in a soilmixture containing vermiculite (60%),perlite (30%) and coco peat (10%) v/v. after 2 months,100% of plants survived andtransferred to the greenhouse.