Isolation and characterization of a novel acidophilic,halotolerant and cold-adaptive cellulase (CMCase) from a native isolate Bacillus sp.

A cold-adaptive acidic cellulase enzyme was produced by native bacillus sp. tg-1 from adana-turkey and the cellulase producing bacteria and the enzyme was characterized. in sds-page analysis the enzyme showed single bond of 52.4 kda. the cellulase tg-1 had a ph optimum of 4.6 and displayed maximum activity at 45°c. the enzyme was stable at ph ranging from 4.0 to 9.0 with more than 85% of original activity for 24h. also,the enzyme showed 100% stability at 20-40°c for 60min and exhibited more than 63% of maximum activity in different nacl concentrations. the enzyme showed up to 70% stability against detergents and chelators,while it was strongly inhibited by urea,h2o2,pmsf and 1,10-phenanthroline and induced by β-mercaptoethanol and mncl2. tlc analyses of the hydrolysis of carboxymethyl cellulose (cmc) by the enzyme,revealed the presence of maltose and etc.