In Silico and Expression analysis of URG-4/URGCP Gene in Different Cancer Cells

Upregulated gene 4 (urg-4/urgcp) located in chromosome 7 (7p13),was originally suggested as one of the hbxag upregulated genes in hepatoma cells and implicated in tumorogenesis process in different cells. in this study we analyzed urg4/urgcp expression at mrna and protein level in various cancer cell lines namely mg-63 (human osteosarcoma),saos-2 (osteosarcoma),mcf-7 (human breast carcinoma),hela (human cervical cancer),pc3 (human prostate cancer),du-145 (human prostate cancer),ht-29 (human colon carcinoma),hl-60 (human myeloid leukemia) and ishikawa (human endometrial adenocarcinoma). the maximum urg-4/urgcp expression was obtained in mg-63 cells (nearly 8.0 fold). it was investigated that hl-60 cells were expressed the maximum protein level of urg-4/urgcp gene (nearly two-fold). bioinformatics analysis of urg-4/urgcp variants on the mrna and protein sequences showed that urg-4/urgcp has 5 variants (variant-1 (3609 bp; 922 aa),variant-2 (3831 bp; 888 aa),variant-3 (3036 bp; 931 aa),variant-4 (3957 bp; 888 aa) and variant-5 (4058 bp; 888 aa). variant-2,-4 and -5 encode the same protein with 888 amino acids.. the expression of variant-2 and -4 of urg-4/urgcp was detected in ht-29,pc3,du-145,mcf-7 and hep3b cells at mrna level using rt-pcr strategy.