isolation and characterisatıon of acidic cellulase enzyme from native bacillus sp. s-41 strain

Acidic cellulase producing bacillus sp. s-41was screened from soil samples in ardicli village, tarsus, turkey and was optimized for the production of extracellular cellulolytic enzyme (cmcase). using sds-page one band is found with molecular mass 53.7 kda. the partially purified enzyme showed optimally activity at ph 4.0 (average 88%) and 50°c and it was stable from ph 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0 after incubating at 37 °c for 24 hour 82%, 96%, 94%, 92%, 90%, 81% and 70%, respectively. enzyme retained its original activity at 50, 60 and 100°c for 60 min 100%, 93%, 70%, respectively. while the enzyme activity was increased in the presence of (1% v/v) β-merkaptoethanol 145% , edta (5mm) 64%, cacl2 (5mm) 58%, zncl2 (5mm) 59%, na-sulfite (5 mm) 60%, 1,10-phenantroline (5mm) 53%, mncl2 pmsf (3mm) 55%, tritonx100 (1% v/v) 61%, sds (1% v/v) 60% and urea (8m) 62% were inhibited respectively. according to these results, bacillus sp. s-41 cellulase enzyme showed acid active and stable, meso-thermophile (between at 40-90 °c, 88% active), highly thermostable and metallo-enzyme properties. therefore it has the potentials to use in cellulose, beverage, prebiotic, feed and food industries areas, bioremediation applications and second generation bioethanol production.