Submerging faba beans in water improve in vitro rooting of microprpagated shoots

A reliable shoot regeneration system for faba bean (vicia faba l.) has not been reported to date to build a strong base for genetic transformation in broad bean. the present work reports a rapid,reproducible and proficient regeneration system for faba bean utilizing plumule and embryonic axis explants on ms medium containing 0.25 mg/l bap +0.50,0.25,0.10,0.05,0.00 mg / l naa following submerging of the seeds in distilled water for 24 hours. the explants showed a fast regeneration in 15 days time. these shoots were rooted on ms medium containing 1 mg/l iaa (15 d) and acclimatised on soil peat moss (10 days). the plantlet regeneration reported in this study accounts for effective micropropagation of faba bean. this protocol establishes a strong foundation and fundamental for future genetic transformation studies in faba beans under in vitro conditions.