association between promoter polymorphisms of il-1β, il-4 and il-6 genes and a viral load infected women with human papillomavirus

Background: the purpose of this study was to investigate the association between il-4 -589c>t, il-6 -174g>c, il-1β -31t>c and il-1β -511c>t genes polymorphism with high concentrations of human papillomavirus (hpv), and the influence of gene-gene interactions on persistent human papillomavirus infection. methods: in this study, 101 infected women with high hpv viral load and 93 healthy women were involved in a case-control study. genotyping of snps for il-4 -589c>t, il-6 -174g>c, il-1β -31t>c and il-1β -511c>t genes was carried out by allele-specific pcr. quantitative analysis of hpv-dna was performed by amplisens hpv hcr genotype-titer software. gene-gene interactions were analyzed using multifactor dimensionality reduction (mdr) algorithm. haplotype interactions were analyzed by haploview 4.2 tool. results: the study of single individual snps in promoters of il-4 -589c>t, il-6 -174g>c and il-1β -31t>c genes did not reveal statistically significant difference in genotypes and allele frequencies among women with high hpv viral load and control group. the frequency of -511t allele and tt genotype of the il-1β gene in case group was significantly higher than the one in control group (or=1.71, p=0.012 and or=2.02, p=0.046, respectively). haplotype analysis revealed that -511c/-31t haplotype for il-1β gene is significantly less common among women with high hpv viral load (p=0.018). conclusion: the haplotype -511c/-31t for il-1β gene is associated with a protective effect against increasing hpv viral load. the frequencies of -511t allele and -511tt genotype of the il-1β -511c>t were significantly higher among women with hpv in comparison to control group.