Derivation of Adipocytes from Human Endometrial Stem Cells (EnSCs)

Background: due to increasing clinical demand for adipose tissue, a suitable cell for reconstructive adipose tissue constructs is needed. in this study, we investigated the ability of human endometrial-derived stem cells (enscs) as a new source of mesenchymal stem cells to differentiate into adipocytes. enscs are the abundant and easy available source with no immunological response, for cell replacement therapy. methods: singlecell suspensions of enscs were obtained from endometrial tissues from 10 women experiencing normal menstrual cycles, and were cultured at clonal density (10 cells/cm2) or limiting dilution. endometrial mesenchymal stem cell markers were examined flow cytometry. these cells were treated with adipogenic- inducing medium for 28 days. the adipogenic differentiation of the ensc was as- sessed by cellular morphology and further confirmed by oil red o staining and rt- pcr. the bm-msc differentiated into adipocytes in the presence of adipogenic stimuli for 3 weeks. results: the flow cytometric analysis showed that the cells were positive for cd90, cd105, cd146 and were negative for cd31, cd34.we showed that the key adipo- cytes marker ppara was expressed in mrna level after 28 days post treatment (pt). conclusion: according to our finding, it can be concluded that enscs represent a useful in vitro model for human adipogenesis, and provide opportunities to study the stages prior to commitment to the adipocyte lineage.