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expression analysis of the crisp2, catsper1, pate1 and semg1 in the sperm of men with idiopathic asthenozoospermia
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نویسنده
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heidary zohreh ,zaki-dizaji majid ,salimi nejad kioomars ,khorram khorshid hamid reza
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منبع
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journal of reproduction and infertility - 2019 - دوره : 20 - شماره : 2 - صفحه:70 -75
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چکیده
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Background: the purpose of this study was to analyze the expression level of crisp2, catsper1, pate1 and semg1 genes in the sperm of men with asthenozoospermia (azs). azs is a cause of infertility in men in which the motility of the sperm is reduced. so far, a few genes have been associated with azs; however, in most of the cases, its molecular etiology is unclear. methods: a total of 35 subjects with idiopathic azs and 35 fertile and healthy men as control were included. in study after total rna extraction and cdna synthesis, relative quantification was performed. b2m was used as the normalizer gene and fold change was calculated by 2−δδctmethod. mann-whitney test was used to compare the expression levels between the case and control groups with significance level of p<0.05. results: our results showed that crisp2 (p=0.03) and semg1 (p=0.03) were significantly down- and up-regulated in azs men respectively compared to the controls. but catsper1 and pate1 did not show significant changes. conclusion: down-regulation of crisp2 and up-regulation of semg1 were associated with azs, which could be suggested as the potential candidate genes for the development of a diagnostic marker or potentially for more studies for treatment of azs.
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کلیدواژه
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asthenozoospermia ,catsper1 ,crisp2 ,gene expression ,male infertility ,pate1 ,realtime pcr ,semg1 ,sperm motility
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آدرس
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search resultsweb resultsacecr.iracecr.iracecr.ir. contact · فارسی · عربی · english. toggle navigation. home · about acecr .... prev. next. acecr r&d complex · acecr r&d complex ...academic center for education, culture and research (acecr), reproductive biotechnology research center, avicenna research institute, iran, academic center for education, culture and research (acecr), reproductive biotechnology research cente, avicenna research institute, iran, academic center for education, culture and research (acecr), reproductive biotechnology research cente, avicenna research institute, iran, academic center for education, culture and research (acecr), reproductive biotechnology research cente, avicenna research institute, iran. university of social welfare and rehabilitation sciences, genetics research center, iran
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پست الکترونیکی
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hrkk1@uswr.ac.ir; h.khorramkhorshid@ari.ir
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Authors
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