The Study of Association Between Polymorphism of Tnf-Α Gene’S Promoter Region and Recurrent Pregnancy Loss

Background: according to the literature review, polymorphisms of tumor necrosis factor alpha’s (tnf-α) promoter region are probably the genetic risk factors of recurrent pregnancy loss. this study has investigated five single nucleotide polymorphisms in the tnf-α gene’s promoter region to evaluate their relationship with recurrent pregnancy loss disorder. methods: blood samples were taken from 65 women with recurrent pregnancy loss (case group) and 65 healthy women with a history of successful pregnancy (control group). polymerase chain reaction with high resolution melting (pcr-hrm) analysis was done to determine the promoter region of -308g/a, -850t/c, -238g/a, -1031t/c and -863a/c tnf-α polymorphisms. the data were assessed using logistic regression models. p values less than 0.05 were considered statistically significant. results: significant associations were found between recurrent pregnancy loss and -863c/a (p=0.000), -308g/a (p=0.045), and -238g/a (p=0.034) polymorphisms. tnf-α polymorphisms of -863c and -238g may be susceptible factors of recurrent pregnancy loss cases. the -308g polymorphism has an important role in maintaining pregnancy. conclusion: the -863c/a and -238g/a tnf-α polymorphisms are possible genetic risk factors of recurrent pregnancy loss and might be its predictive markers.