Techniques for Ovarian Tissue, Whole Ovary, Oocyte and Embryo Cryopreservation

In recent years, preservation of fertility in women has been of great importance,especially in patients exposed to deleterious conditions on fertility. thus, cryopreservationof human gametes, embryos and ovarian tissue has become an essential partof assisted reproduction. this approach limits the number of embryos transferred,while supernumerary oocytes and/ or embryos can be used in subsequent treatmentcycles. furthermore, cryopreservation reduces the potential risk of hyperstimulationsyndrome. cryopreservation is carried out by two techniques; the slow freezingmethod, and the more recent rapid procedure called vitrification technology. recentlydue the success and simplicity of vitrification, the balance between those two methodshas been changed in advantage of vitrification. the use of slow freezing method hasbecome controversial due to its difficulties, expense and respective low success ratesin artificial reproduction. therefore, vitrification seems to win the battle and will bethe cryopreservation method of the future