synthesis of alaninemethylester and n-(4-aminobenzyl)-9hpurin-6-amine doped to polyaniline nanoparticles and study on their interactions with ds-dna by fluorescence spectroscopy

In  this  paper,  two  major  projects  have  been  successfully  accomplished: firstly,  we  introduced a novel  and  highly  efficient  route which  was developed  for  the synthesis  of  polyaniline  (pani) nanoparticles by using potassium persulfate. structure of nano pani was characterized by fouriertransform  infrared  (ft-ir),  proton  nuclear  magnetic resonance  (1h-nmr) and  elemental analysis  and  the  surface  morphology  of  them was  studied  by scanning  electron  microscopy (sem).secondly,  new  nano-biosensors  have been synthesized  by  using  a  simple  and  efficient method, from the reaction of nano pani with the biocompatible branches, such as alaninemethyl ester and n-(4-aminobenzyl)-9h-purin-6-amine as a nucleobase and succinic anhydride as a spacer. the  structures  of  6  and  7  were  confirmed  by  ft-ir  and 1h-nmr.  the  interaction  between alaninemethylester and nucleobase doped to nano pani with short oligo phosphate chains of dsdna (45 base pairs), was studied by fluorescence spectroscopy. the results showed that existence of dopants in the structure of nano pani increases the interactions with dna and this caused the decrease in the fluorescence emission intensity.