ultrasound-assisted emulsification microextraction followed by gas chromatography as an efficient and sensitive technique for determination of olanzapine in biological samples

Ultrasound-assisted  emulsification  microextraction  (usaeme)  and  gas  chromatography – flame ionization detection (gc-fid) was presented for the extraction and determination of olanzapine in human urine and plasma samples. chlorobenzene at microliter volume level as an extraction solvent without  disperser  solvent  was  used.  the  main advantages  of  this  method  are  high  speed,  high recovery,  good  repeatability  and  extraction  solvent  volume  at  µl  level.  the  effect  of  several variables such as type and volume of extraction solvent, ultrasonication time, centrifugation time, salt addition, etc. were evaluated, carefully. in the optimum conditions, the calibration curve was linear in the range of 70-2000 µg l-1 with the detection limit of 20 µg l-1. the relative standard deviation  (r.s.d.)  for  the  five  replicate  measurements  of  olanzapine  was  4.6  %.  usaeme combined with gc-fid is a fast, simple and efficient method for the determination of olanzapine in human urine and plasma samples.