Antibacterial and Cytotoxicity Effects of TiO2-Grafted Cellulose Nanocomposite

Tio2-grafted cellulose bio-nanocomposite (cell.com) was successfully prepared by combiningmodified cellulose and tio2 nanoparticles via copper-catalyzed alkyne-azide click reaction. theantibacterial and cytotoxicity effects of cell.com containing cellulose, triazole ring and tio2moieties are reported in this work. the antibacterial activity of cell.com was investigated againstgram positive (s. aureus and b. subtilis) and gram negative (e. coli and p. aeruginosa) bacteria. theantibacterial results indicate that the cell.com exhibits a wide range of antibacterial activity againstboth gram positive and gram negative bacteria which is due to the presence of tio2 and triazolering moieties in its structure. in addition, the cytotoxicity effect of cell.com was studied in vitro oncervical cancer (hela), breast carcinoma (mcf-7), sarcoma osteogenic (saos) and fibroblast cellsby mtt assay. the cell viability results show that the cell. com diminishes relatively cancer cellsgrowth in a dosage and time dependent way probably through triazole moiety, while no apparentcytotoxicity effect is observed on the normal fibroblast cells.