Microextraction and Determination of Diclofenac in Biological Samples using Hollow Fiber Liquid Phase Microextraction Technique Coupled with HPLC-UV

In this study, hollow fiber liquid phase microextraction (hf-lpme) coupled with highperformance liquid chromatography (hplc) with uv detection was applied for preconcentration and determination of diclofenac sodium in biological fluids. parameters affecting the extraction process including ph of donor phase and acceptor phase, type of extraction solvent, stirring rate, extraction time, and salt addition were studied and optimized. under the optimized conditions donor phase ph=3, acceptor phase ph=11.7, extraction solvent n-octanol, stirring rate =750 rpm, extraction time=27 min and without the addition of salt, enrichment factors up to 170 were achieved and the relative standard deviation (rsd %) of the method was within the range of 2- 3%. the method was successfully applied to determine the concentration of diclofenac sodium in the biological samples and satisfactory results were obtained.