Mediated Electrochemical Study on Microperoxidase-11(MP-11)/ DNA System Film Using a New Mediator

Abstract:direct redox conversion of microperoxidase-11(mp-11) was studied and demonstrated by cyclic voltammetry on a gold electrode modified by a self- assembled monolayer (sam) of a new sulfur containing compound (6-amino-5-nitroso-4-hydroxy-1,3-diaza-5- cyclohexene-2-thioone) and dna film. the results showed a pair of well defined, reversible cyclic voltammetric peaks. it thus obtained at an (mp-11)-s-compound-dna modified au electrode. its formal potential, é0 is 0.224 v for 0.1mol/lit phosphate buffer solution (pbs), ph 7 at a scan rate of 500 mv/s. the formal heterogeneous electron transfer rate constantof mp-11 redox conversion on the s-compound and dna modified electrode was 2.14 s-1. dna can be considered as a charge carrier to facilitate electron transfer between the enzyme and electrode.