Development and Validation of a Reversed Phase HPLCMethod for Simultaneous Determination of Amlodipineand Telmisartan in Pharmaceutical Dosage Form

Abstract:a simple, accurate and precise method for the simultaneous determination of amlodipine and telmisartan in bulk drug and pharmaceutical dosage has been developed by rp-hplc method. separation was performed on a 5μm nucleodur® c18 column (250 х 4.6mm id) with acetonitrile: phosphate buffer at ph 4.5 (60:40v/v) in isocratic elution in less than 9 min with a flow rate of 1.3 ml min-1. good sensitivity for all analytes was observed with uv detection at 238 nm. the method allowed quantification over the 1-11 μg ml-1 range for amlodipine and 8-80 μg ml-1 range for telmisartan. the method has been applied, without any interference from excipients or endogenous substances, for the simultaneous estimation of these two compounds in bulk drug and in tablets.