Molecular epidemiology and phylogenetic analysis of Dengue virus type-1 and 2 isolated in Malaysia

Objective: detection of different serotypes of dengue virus and provide information on origin, distribution and genotype of the virus. methods: dengue virus serotypes identified as den-1 and den-2 were amplified and sequenced with e gene. the consensus sequences were aligned with references e gene sequences of globally available genbank. phylogenetic analysis was performed using neighbor-joining and kimura 2-parameter model to construct phylogenetic tree. results: a total of 53 dengue virus isolates were positive, of which 38 (71.7%) were denv-1 and 15 (28.3%) were denv-2. phylogenetic tree of denv-1 and denv-2 showed that the isolates were clustered in genotype i and cosmopolitan genotype, respectively considered the predominant genotypes in southeast asian countries. the molecular epidemiology genotype i denv-1 and cosmopolitan genotype denv-2 have been co-circulating in klang valley areas, malaysia without shifting of genotype. conclusion: the study reveals that denv-1 and denv-2 have been circulating in malaysia. the isolates are clustered in genotype 1 and cosmopolitian genotype, respectively. the study results would help in planning for prevention and control of dengue virus in malaysia.