Development and Validation of HPLC Method for Simultaneous Quantification of Prifinium Bromide/Paracetamol Combination in Pharmaceutical Formulations

A simple, rapid, accurate and precise hplc method was developed and validated for the simultaneous quantification of prifinium bromide and paracetamol combination in pharmaceutical dosage form. the separation was achieved on a normal phase-nitrile (cn) column. the mobile phase consisted of 30 mm ammonium acetate buffer–acetonirtile (50%:50%, v/v) at ph = 6.5. the uv detection was carried out at a wavelength of 254 nm. a linear response was observed over a concentration range 3–50 μg/ml for prifinium bromide and paracetamol. the limit of detection for prifinium bromide was 0.75 μg/ml and its limit of quantification was 2.30 μg/ml. for paracetamol, the limit of detection was 0.64 μg/ml while its limit of quantification was 1.60 μg/ml. the method was validated for linearity, accuracy, precision and robustness. the validated method was applied to the analysis of prifinium bromide and paracetamol in commercial riabal®compound tablets.