Preparation and HPLC Analysis of the Natural Pigments Obtained from Buckthorn (Rhamnus petiolaris Boiss) Dye Plants

In this work, the buckthorn natural pigments, namely aluminium, tin and iron-buckthorn were obtained by the reaction of kal(so4)2.12h2o, sncl2.2h2o and feso4.7h2o solutions with buckthorn (rhamnus petiolaris boiss) berries. a reversed-phase high performance liquid chromatography (hplc) with diode-array detection (dad) method was utilized for the identification of the buckthorn natural pigments. the extraction of dyestuffs from the natural pigments was carried out with hydrochloric acid / methanol / water (2:1:1; v/v/v) solution. according to the results of hplc analysis of the natural pigments, it was observed that both rhamnetin and emodin that present in the pigments were precipitated by al(iii) whereas only emodin was precipitated by sn(ii) and fe(ii). cielab values of the natural pigments were measured.