Bivariate Analysis for the Determination of Paracetamol and Caffeine in Drug Formulations

The uv absorption spectrum of paracetamol/caffeine combination shows strongly overlapped features. bivariate spectrophotometric analysis was applied to quantify a paracetamol/caffeine combination in commercial drug samples. the proposed method was tested according to ich guidelines and compared to the validated hplc-uv method. the results obtained by the bivariate method are in a good agreement with the labeled contents. statistical evaluation using student’s t-test and f-test showed no significant differences between the results obtained by the bivariate method and the hplc method for commercial drug samples. the bivariate spectrophotometric method is rapid, precise and accurate; it could effectively be an alternative method to hplc.