اثر برخی نانوذرات و کودهای بیولوژیک بر اجزای فلورسانس ‏کلروفیل و برخی صفت‌های فیزیولوژیک تریتیکاله ‏‎(x ‎triticosecale wittmack)‎‏ در سطوح مختلف آبیاری

به‌منظور بررسی تاثیر نانوذرات (نانواکسید آهن و سیلیکون) و کودهای بیولوژیک (آزوسپریلیوم لیپوفروم و سودوموناس پوتیدا) بر اجزای فلورسانس کلروفیل و برخی صفت‌های فیزیولوژیک تریتیکاله در سطوح مختلف آبیاری، آزمایشی به‌شکل فاکتوریل در قالب طرح پایۀ بلوک‌های کامل تصادفی در سه تکرار اجرا شد. فاکتورهای بررسی‌شده، سه سطح آبیاری (آبیاری کامل به‌عنوان شاهد، قطع آبیاری در 50 درصد مراحل چکمه‌ای‌شدن (آبستنی) و سنبله‌دهی به‌ترتیب به‌عنوان محدودیت شدید و ملایم آبی)، کاربرد کودهای زیستی (آزوسپریلیوم لیپوفروم و سودوموناس پوتیدا) در چهار سطح و محلول‌پاشی نانوذرات (نانواکسید آهن و سیلیکون) در چهار سطح بودند. کاربرد توام باکتری‌های محرک رشد و محلول‌پاشی نانوذرات در شرایط آبیاری کامل، شاخص کلروفیل (50.23 درصد)، محتوای نسبی آب (43.97 درصد)، هدایت روزنه‌ای (36.78 درصد)، عملکرد کوانتومی (47.38 درصد)، فلورسانس حداکثر (34.84 درصد)، فلورسانس متغیر (98.49 درصد) و عملکرد دانه (43.28 درصد) را در مقایسه با قطع آبیاری در مرحلۀ آبستنی و به‌کارنبردن کودهای بیولوژیک و نانوذرات افزایش داد. کاربرد توام نانوذرات و باکتری‌های محرک رشد، شاخص کلروفیل، محتوای نسبی آب، هدایت روزنه‌ای، عملکرد کوانتومی، فلورسانس حداکثر، فلورسانس متغیر و عملکرد دانه در هر دو سطح آبیاری کامل و محدودیت شدید آبی را افزایش داد، ولی نشت الکترولیت و فلورسانس حداقل را کاهش بخشید؛ همچنین در شرایط محدودیت شدید آبی یا قطع آبیاری در مرحلۀ آبستنی، کاربرد توام نانوذرات و باکتری‌های محرک رشد شاخص کلروفیل (7.74 درصد)، محتوای نسبی آب (10.99 درصد)، هدایت روزنه‌ای (22.46 درصد)، عملکرد کوانتومی (15.2 درصد)، فلورسانس حداکثر (26.54 درصد)، فلورسانس متغیر (75.61 درصد) و عملکرد دانه (28.59 درصد) را نسبت به شرایط به‌کارنبردن نانوذرات و کودهای بیولوژیک در همین سطح از سطوح آبیاری افزایش داد. به‌طورکلی، کاربرد توام کودهای بیولوژیک و نانوذرات به‌واسطۀ بهبود صفت‌های فیزیولوژیک گیاه، عملکرد دانۀ بهتری را در سطوح مختلف آبیاری نشان داد.

the effect of some nanoparticles and biofertilizers on chlorophyll ‎fluorescence components and some physiological traits of triticale ‎‎(triticosecale wittmack) at different irrigation levels

in order to investigate the effects of nanoparticles) nano fe-si oxide) and biofertilizers (azospirillum lipoferum and pseudomonas putida) on chlorophyll fluorescence components and some physiological traits of triticale at different levels of irrigation, an experimental as the factorial study was conducted based on randomized complete block design with three replications. the studied factors included three levels of irrigation (full irrigation as control, irrigation withholding at 50% of booting and heading stages as severe, and moderate water limitation, respectively), application of biofertilizers (azospirillum  lipoferum and pseudomonas putida) in four levels, and nanoparticles foliar application (nano iron oxide and nano silicon oxide) at four levels. both applications of plant growth-promoting rhizobacteria and nanoparticle foliar application under full irrigation increased chlorophyll index (50.23%), relative water content (43.97%), stomatal conductance (36.78%), quantum yield (47.38%), maximum fluorescence (34.84%), variable fluorescence (98.49%), and grain yield (43.28%) in comparison to no application of biofertilizers and nanoparticles under irrigation withholding at booting stage. both applications of plant growth-promoting rhizobacteria and nanoparticles increased the chlorophyll index, relative water content, stomatal conductance, quantum yield, maximum fluorescence, variable fluorescence and grain yield in both levels of full irrigation and severe water limitation, but decreased electrolyte leakage and minimum fluorescence. in addition, severe water limitation or irrigation withholding at the booting stage increased chlorophyll index (7.74%), relative water content (10.99%), stomatal conductance (22.46%), quantum yield (15.2%), maximum fluorescence (26.54%), variable fluorescence (75.61%) and grain yield (28.59%) in comparison to no application of biofertilizers and nanoparticles at the same irrigation level. generally, the application of biofertilizers and nanoparticles showed a better performance at different irrigation levels due to the improvement of physiological traits.introductiontriticale is a human-made crop, being a hybrid by cross-fertilization of wheat (triticum spp.) and rye (secale spp.). in general, triticale combines the high yield potential of wheat with the biotic and abiotic stress tolerance of rye, making it more suitable for production in marginal areas (acidic, saline, or soils with heavy metal toxicity) (cantale et al. 2016). in arid and semi-arid regions, drought stress as the main factor and salinity stress as a secondary factor decrease plant growth and yield. water limitation can damage pigments and plastids, and educe chlorophyll index, stomatal conductance, quantum yield, and relative water content. several strategies have been developed in order to decrease the toxic effects caused by environmental stresses on plant growth. among them, the use of bio-fertilizers such as plant growth-promoting rhizobacteria (pgpr) and also nanoparticles such as nano iron-silicon oxide plays a very important role in yield improvement. inoculation of plants with native suitable microorganisms may decrease the deleterious effects of environmental stresses and increase stress resistance of plants by various mechanisms, including synthesis of phytohormones such as auxins, cytokinin and gibberellins, solubilization of minerals like phosphorus, production of siderophores and increase in nutrient uptake. there is little information on the effects of nanoparticles and biofertilizers on chlorophyll fluorescence components and some physiological traits of triticale at different irrigation levels. therefore, the objective of the present study was to evaluate the effect of nanoparticles (nano fe-si oxide) and biofertilizers (azospirillum  lipoferum and  pseudomonas putida) on chlorophyll fluorescence components and some physiological traits of triticale at different levels of irrigation. materials and methodsan experiment as factorial was conducted based on a randomized complete block design with three replications at the research farm of the faculty of agriculture and natural resources, university of mohaghegh ardabili in 2021. the experimental factors included irrigation in three levels (full irrigation as control, irrigation withholding at 50% of booting and heading stages as severe, and moderate water limitation, respectively (bbch 43 and 55 respectively), the application of biofertilizers in four levels (no application as control, application of azospirillum, pseudomonas, both application azospirillum + pseudomonas) and nanoparticles foliar application at four levels (foliar application with water as control, nano iron oxide foliar application (1 g.l-1), nano silicon oxide (50 mg.l-1), both application nano iron-silicon oxide). psedomunas and azospirillum were isolated from the rhizospheres of wheat by the research institute of soil and water, tehran, iran. for inoculation, seeds were coated with gum arabic as an adhesive and rolled into the suspension of bacteria until uniformly coated. the strains and cell densities of microorganisms used as pgpr in this experiment were 1×108 colony forming units (cfu). in each plot, there were 5 rows with 2 m long. in each experimental plot, two beside rows and 0.5 m from the beginning and end of planting lines were removed as margin, and measurements were done on 0.2 m2 for grain yield. the used nano silicon-iron oxide had an average particle size of less than 30 nm and the special surface of particles was more than 30 m2.g-1. they were the product of nanomaterial us research which was provided by pishgaman nanomaterials company of iran. nano silicon oxide and nano silicon oxide powder were added to deionized water and placed on ultrasonic equipment (100 w and 40 khz) on a shaker for better solution. foliar application of nano silicon and nano iron oxide was done in two stages of period growth bbch 21 and 30. chlorophyll index was calculated by a chlorophyll meter (spad-502; konica minolta sensing, inc., japan). rwc and el were measured according to the method of kostopoulou et al. (2010) and farooq and azam (2006), respectively. quantum yield was measured on flag leaves by the uppermost fool expanded leaf using a fluorometer (chlorophyll fluorometer; optic science-os-30 u.s.a.). analysis of variance and mean comparisons were performed using software package sas v9.12. the main effects and interactions were compared by lsd (least significant difference) test at the 0.05 probability level, using the sas version 9.1.  results and discussionthe results showed that both applications of plant growth-promoting rhizobacteria and nanoparticle foliar application under full irrigation increased chlorophyll index (50.23%), relative water content (43.97%), stomatal conductance (36.78%), quantum yield (47.38%), maximum fluorescence (34.84%), variable fluorescence (98.49%), and grain yield (43.28%) in comparison to no application of biofertilizers and nanoparticles under irrigation withholding at booting stage. both applications of plant growth-promoting