ریزازدیادی گیاه ‏haworthia limifolia‏ با استفاده از ‏تنظیم‌ کننده‌های رشد گیاهی

کاکتوس (haworthiopsis limifolia)، بومی آفریقای جنوبی، یک گیاه زینتی علفی از خانواده asphodelaceae است. این گیاه چند ساله گوشتی به دلیل خواص دارویی از جمله خواص ضد باکتریایی و ضد قارچی شناخته شده است. تولید و کشت این گیاه به طور سنتی با عملکرد کم و زمان بر بوده و پاسخگوی نیاز بازار نیست و تولید کننده برای تولید انبوه با مشکل مواجه است. برای حل این مشکل از روش های جدید تکثیر مانند کشت بافت استفاده می شود. هدف از مطالعه ما بررسی و شناسایی روشی برای ضد عفونی کردن کشت بافت در شرایط آزمایشگاهی هارورتیا و سپس بررسی اثرات سطوح مختلف تنظیم‌کننده‌های رشد شامل bap (بنزیل آمینوپورین) و iba (ایندول بوتیریک اسید) بر ریزازدیادی این گیاه بود. گیاه. نتایج ما نشان داد که محلول هیپوکلریت سدیم 1.5 درصد به مدت 10 دقیقه بهترین تیمار برای ضدعفونی کشت های جدا شده بود. نتایج ما نشان داد که بیشترین تعداد ساکولنت (10) مربوط به 1.5میلی‌گرم در لیتر bap بود. مقایسه میانگین نشان داد که بهترین تیمار برای ریشه زایی (1.5 میلی‌گرم در لیتر iba) بود. حداکثر تعداد ساکولنت ها از 1.5 میلی گرم در لیتر bap به دست آمد. بر اساس نتایج، bap نسبت به iba تاثیر معنی‌داری بر ریشه‌زایی، ساچمه‌زنی و مکیدن کاکتوس‌ها داشت.

micropropagation of haworthia limifolia using plant growth ‎regulators

haworthiopsis limifolia, a cactus native to south africa, is one of the herbaceous ornamental plants belonging to the asphodelaceae family. this succulent perennial plant is known for its medicinal properties, including antibacterial and antifungal properties. the production and cultivation of this plant are traditionally associated with low yield and time consumption, which do not meet any needs of the market, and the producer faces problems with mass production. to solve these problems, new propagation methods, such as tissue culture, are used. the objectives of this study were to introduce a novel protocol for disinfecting hawthorn for in vitro tissue culture and investigate the effects of different levels of growth regulators, including bap (benzyl amino purine) and iba (indole butyric acid), on the micropropagation of this plant. the results showed that 1.5% sodium hypochlorite solution for 10 minutes was the best treatment for the disinfection of the isolated cultures. in addition, our results indicated that the highest number of succulents (10) belonged to the treatment of 1.5 mg /l bap. according to the mean comparison, the best treatment for rooting was 1.5 mg/l of iba.  the 1.5 mg/l concentration of bap led to the maximum number of succulents. moreover, bap had a more significant effect than iba on cacti’s rooting, shooting, and suckering.introduction the haworthia limifolia, with the scientific name haworthia limifolia, is an ornamental, herbaceous, and perennial plant from the asphodelaceae family and tends to fall under the category of succulent cactus plants. this particular plant originates from south africa and is commonly grown for decorative purposes. it is also known for its medicinal properties, which include antibacterial and antifungal benefits. haworthia limifolia is a highly prized plant, sought after for its unique and stunning appearance, featuring two distinct colors, and it is often considered an ornamental plant. this plant’s compact size allows for easy cultivation in a small pot or a lovely terrarium alongside other decorative succulents. in addition, in the past years, haworthia limifolia, like aloe vera plants, has been widely considered in traditional medicine among the natives of the region. traditionally, the yield and breeding of this plant are low and time-consuming, which fails to meet market demands. as a result, the producer encounters difficulties in mass production. modern methods, like tissue culture, are used to solve this problem. the success rate of micropropagation depends on multiple factors, including explant type, nutrients, plant growth regulators, additives, temperature, light intensity, and duration.