جداسازی و شناسایی بیوشیمیایی و مولکولی باکتری های پروبیوتیک از شیر و ماست سنتی گاومیش شهرستان خوی

The aim of this study was to isolate and identify the probiotic bacteria from microbial flora of buffalo milk and its traditional yogurt in khoi city. to achieve this goal, the lactic acid bacteria were isolated by phenotypic methods (cellular morphology, gram staining, catalase test, biochemical tests including growth in different temperatures, various concentrations of salt and fermentation of different types of sugar) and their probiotic potential (resistant to stomach acid and bile salts) were evaluated. then, to identify more accurately, the 16s rrna gene of bacteria were replicated with pairs of specific primers and then the purified pcr product was sent for gene sequencing. at the end, 13 strains of lactobacilli and 6 strains of enterococci were reported as the natural microbial flora with probiotic potential in khoi city. these bacteria provide the good quality of the dairy products in those areas and can be used in industrial manufacture of dairy products