خالص‌سازی ایزومرهای اسید لینولییک مزدوج از طریق استریفیکاسیون انتخابی با لیپاز کاندیدا رگوزا

Ability of candida rugosa for separation of two main conjugated linoleic acid (cla) isomers including 9-cis,11-trans and 10-trans,12-cis was investigated in the mixture of free fatty acids of these isomers and l-menthol. in order to effectively separate two mentioned isomers, optimization of reaction conditions was conducted using response surface methodology (rsm). after data analysis by d-optimal design, the best reaction conditions for maximum esterification of 9-cis,11-trans isomer with l-menthol after just one step esterification were as follows: reaction time 23.1 h, temperature 32.6°c, enzyme amount 135.4u, molar ratio of cla oil to l-menthol at 1:1.7 and ph at 7.7. at this optimum point, the lowest amount of c9, t11-cla isomer (6.7%) was observed in free fatty acids fraction. this effective process for purification of cla isomers using l-menthol could be applicable to produce food supplements.