بررسی ویژگی بالقوه پری بیوتیکی زایلوالیگوساکاریدهای تولید شده به روش آنزیمی ازهسته خرما

زﻣﯿﻨﻪ ﻣﻄﺎﻟﻌﺎﺗﯽ: ﻫﺴﺘﻪ ﺧﺮﻣﺎ ﺣﺎوی ﻣﻘﺎدﯾﺮ ﺑﺎﻻﯾﯽ ﻓﯿﺒﺮاﺳﺖ ﮐﻪ ﺑﺎ اﺳﺘﺨﺮاج اﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪﻫﺎی آن ﻣﯽﺗﻮان ﺑﻪ ﻣﻮاد ﻓﺮاﺳﻮدﻣﻨﺪی ﻣﺎﻧﻨﺪ ﭘﺮیﺑﯿﻮﺗﮏﻫﺎ دﺳﺖ ﯾﺎﻓﺖ. ﻫﺪف : ﻫﺪف اﯾﻦ ﺗﺤﻘﯿﻖ، اﺳﺘﺨﺮاج زاﯾﻼن از ﻫﺴﺘﻪ ﺧﺮﻣﺎ، ﺗﺒﺪﯾﻞ آﻧﺰﯾﻤﯽ آن ﺑﻪ زاﯾﻠﻮاﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪﻫﺎ و ﺑﺮرﺳﯽ ﻗﺎﺑﻠﯿﺖ ﭘﺮیﺑﯿﻮﺗﯿﮑﯽ آﻧﻬﺎ در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﭘﺮیﺑﯿﻮﺗﯿﮏ ﺗﺠﺎری (ﻓﺮوﮐﺘﻮاﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪ)اﺳﺖ. روش ﮐﺎر: زاﯾﻼن ﻫﺴﺘﻪ ﺧﺮﻣﺎ (رﻗﻢ ﮐﺒﮑﺎب) اﺳﺘﺨﺮاج و ﺳﭙﺲ ﺑﺎ اﺳﺘﻔﺎده از دو آﻧﺰﯾﻢ زاﯾﻼﻧﺎز ﺗﺠﺎری ﺑﻪ ﻧﺎم ﭘﻨﺘﻮﭘﺎنﻣﻨﻮﺑﯽﺟﯽ و ورون191 آﺑﮑﺎﻓﺖ ﮔﺮدﯾﺪ. ﮐﻤﯿﺖ و ﮐﯿﻔﯿﺖ زاﯾﻠﻮاﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪﻫﺎی ﺣﺎﺻﻞ، ﺑﻪ وﺳﯿﻠﻪ ﮐﺮوﻣﺎﺗﻮﮔﺮاﻓﯽ ﻣﺎﯾﻊ ﺑﺎ ﮐﺎراﯾﯽ ﺑﺎﻻ ﺗﻌﻮﯾﺾ ﯾﻮﻧﯽ ﺗﻌﯿﯿﻦ ﮔﺮدﯾﺪ. ﺑﺮای ﺳﻨﺠﺶ ﺑﺮونﺗﻨﯽ ﻫﻀﻢﭘﺬﯾﺮی ﮔﻮارﺷﯽ، زاﯾﻠﻮاﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪﻫﺎی ﺣﺎﺻﻞ از آﺑﮑﺎﻓﺖ، در ﻣﻌﺮض ﻣﺤﻠﻮل ﻫﺎی ﺷﯿﻤﯿﺎﯾﯽ ﻣﺸﺎﺑﻪ ﺷﯿﺮهﻫﺎی ﮔﻮارﺷﯽ ﻗﺮار داده ﺷﺪ. ﻫﻤﭽﻨﯿﻦ اﺛﺮ زاﯾﻠﻮاﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪﻫﺎی ﺣﺎﺻﻞ، ﺑﻪ ﻋﻨﻮان ﻣﻨﺒﻊ ﮐﺮﺑﻦ، ﺑﺮ رﺷﺪ ﺑﯿﻔﯿﺪوﺑﺎﮐﺘﺮﯾﻮم ﺑﯿﻔﯿﺪوم (ptcc1644)، در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮐﻨﺘﺮل ﻣﻨﻔﯽ (ﻣﺤﯿﻂ ﮐﺸﺖ ﺑﺪون ﻣﻨﺒﻊ ﮐﺮﺑﻦ) و ﮐﻨﺘﺮل ﻣﺜﺒﺖ ( ﮔﻠﻮﮐﺰ) و ﭘﺮیﺑﯿﻮﺗﯿﮏ ﺗﺠﺎری ﻣﻮرد ارزﯾﺎﺑﯽ ﻗﺮار ﮔﺮﻓﺖ. ph ﻣﺤﯿﻂ ﻫﺎی ﮐﺸﺖ ﻧﯿﺰ در ﻓﻮاﺻﻞ زﻣﺎﻧﯽ دو ﺳﺎﻋﺖ ﯾﮏﺑﺎراﻧﺪازهﮔﯿﺮی ﺷﺪ. ﻧﺘﺎﯾﺞ: ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ اﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪﻫﺎی ﺣﺎﺻﻞ از آﺑﮑﺎﻓﺖ، ﻣﻘﺎوم ﺑﻪ ﻫﻀﻢ ﺑﻮدﻧﺪ. ﺑﯿﻦ زاﯾﻠﻮاﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪﻫﺎ، ﭘﺮیﺑﯿﻮﺗﯿﮏ ﺗﺠﺎری و ﮔﺮوﻫﻬﺎی ﮐﻨﺘﺮل، از ﻧﻈﺮ اﺛﺮ ﺑﺮ ﺳﺮﻋﺖ رﺷﺪ(µmax) و داﻧﺴﯿﺘﻪ ﻧﻮری ﺑﻌﺪ از 24 ﺳﺎﻋﺖ (od600)، ﺗﻔﺎوت ﻣﻌﻨﯽداری وﺟﻮد داﺷﺖ (0/05 ≤p). اﻣﺎ ﺑﯿﻦ دو زاﯾﻠﻮاﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪ ﺣﺎﺻﻞ از دوآﻧﺰﯾﻢ ﻣﺬﮐﻮر، ﺗﻔﺎوت ﻣﻌﻨﯽداری ﻣﺸﺎﻫﺪه ﻧﺸﺪ (0/05 >p). ﮐﺎﻫﺶ ph ﻣﺤﯿﻂ ﻫﺎی ﺣﺎوی زاﯾﻠﻮاﻟﮕﻮﺳﺎﮐﺎرﯾﺪ در ﺑﯿﻦ ﺳﺎﯾﺮ ﻣﺤﯿﻂ ﻫﺎ (ﻧﺴﺒﺖ ﺑﻪ ﻧﻤﻮﻧﻪ ﮐﻨﺘﺮل ﻣﻨﻔﯽ) ، ﺑﯿﺸﺘﺮﯾﻦ ﺑﻮد ﮐﻪ ﻧﺸﺎن دﻫﻨﺪه ﺗﻮﻟﯿﺪ ﺑﯿﺸﺘﺮ اﺳﯿﺪﻫﺎی آﻟﯽ اﺳﺖ. ﻧﺘﯿﺠﻪ ﮔﯿﺮی ﻧﻬﺎﯾﯽ: ﺑﻪ دﻟﯿﻞ ﻫﻀﻢ ﻧﺎﭘﺬﯾﺮی زاﯾﻠﻮاﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪﻫﺎی ﺑﻪدﺳﺖآﻣﺪه از آﺑﮑﺎﻓﺖ آﻧﺰﯾﻤﯽ زاﯾﻼن ﻫﺴﺘﻪ ﺧﺮﻣﺎ و اﺛﺮ ﻣﺜﺒﺖ ﺑﺮ رﺷﺪ ﺑﯿﻔﯿﺪوﺑﺎﮐﺘﺮﯾﻮم ﺑﯿﻔﯿﺪوم ، ﻣﯽﺗﻮان ﺧﺎﺻﯿﺖ ﺑﺎﻟﻘﻮه ﭘﺮیﺑﯿﻮﺗﯿﮑﯽ ﺑﻪ آﻧﻬﺎ ﻧﺴﺒﺖ داد.

Evaluation of Prebiotic Potential of the Xylooligosaccharides Produced by Enzymatic Method from Date Kernel

The xylan of date kernel variety KabKab, was extracted and then was hydrolyzed by two commercial xylanases called Pentopan mono BG and Veron 191. The xylooligosaccharides (XOS) in the two hydrolyzates were qualified and quantified by HPAECPAD. In order to testing digestibility of the XOS, the hydrolyzates were exposed to the solutions similar gastric juices. Results showed that the XOS are resistand to digestion. Effect of the XOS, as carbon source, on growth of Bifidobacterium Bifidum, Compared with other carbon sources, was determind too. The results showed that maximum growth rate and optical density at time 24 houre (OD24) for the two XOS have a significant difference with two other carbon source and control but no significant difference was observed between the two XOS at 95% possibility. pH of cultures at interval times were determined. Decrease of pH for XOS was more, that indicates more organic acid production. Overall, due to indigestibility of these XOS and their positive effect on Bifidobacterium bifidum, prebiotic property potential can attribute to them.