بررسی نقص های ژنتیکی cvm و bladبا استفاده از سلول‌های سوماتیک شیر و سلول های خوندر جمعیتی از گاوهای هلشتاین

Background:in the present research, molecular detection of bovine leukocyte adhesion deficiency (blad) and complex vertebral malformation (cvm) in a population of holstein cows have been done using milk somatic cells comparing with blood cells. blad characterized by negative affecting on the haematopoietic system via reduced expression of the adhesion molecules on neutrophils. the molecular cause of blad is a single point mutation. cvm characterized by intra-uterine mortality with some disorders. the molecular cause of cvm is also a point mutation.objectives:this study has been done in order to prevention of intra-uterine mortality and comparing of dna extraction by milk or blood.methods:in the first step of our research program, tank milk samples from 50 herds were collected. pcr-rflp was performed to detect a point mutation of both cvm and blad genes. then an affected herd with 120 cows was randomly selected for individual test using blood samples. we showed two cows out of 120 were identified as carriers of this gene.we have done 50 dna extractions and molecular testes in order to detect existence of carriers in 50 herds with total of 10000 cows and we have done also 120 testes for detection of carriers in a herd with 120 cows.results:in these herds, we didn’t find any affected herd with the mutant allele of blad but we found the single point mutation of cvm in 17 different herds.conclusions:in the present study we showed that, detection for all genetic defects using tank milk samples is more economic and easyer than the other methods. because if we assume the average of 200 dairy cows for each herd, in fact we have done 50 testes instead of 10000 in 50 herds. on the other hand, sampling with less stress for cows is always preferable for breeders to blood sampling for purposes like detection of genetic defects or viral diseases.