اثر تزریق داخل تخم‌مرغی ال – گلوتامین بر عملکرد رشد قبل و پس از تفریخ، مورفولوژی روده و پاسخ‌های ایمنی جوجه‌های گوشتی

The aim of this study was to evaluate the effect of in ovo injection of l- glutamine on pre and post hatch growth performance, carcass traits, jejunum morphology and immune responses of broiler chickens. four hundred eighty fertile ross 308 broiler breeder eggs in a completely randomized design were used into 6 experimental groups, 4 replicates of 20 eggs each. treatment groups were: non-injected control, sham control (sterile water), 10, 20, 30 and 40 mg glutamine were dissolved in 1ml sterile water and then inoculated into amniotic fluid of the 17.5 d- old embryo through broad end of the egg. after hatch, 10 chicks per each replicate were distributed in pens and reared up to 42 d of age. the results showed that the hatchling weights and overall daily body weight gain were 3.6% and 3.8% higher in the group given 40 mg glutamine than noninjected control, respectively (p < 0.05). at day 24, relative weight of breast muscle was influenced by embryo feeding of glutamine (p < 0.05), but relative weights of liver, gizzard and heart were not affected (p < 0.05). compared with untreated control, glutamine was enhanced jejunal villi height and crypt depth in 3 and 24 days of post hatch. by 40 mg glutamine injection, the humoral and cell mediated immune responses were improved. the results suggest that in ovo injection of 40 mg l-glutamine into amniotic fluid of growing embryo can improve hatchling weight, average daily gain, morphometric development of the intestinal tract and humoral and cellular immune responses of broiler chickens