اثر افزودن مایع منی به اسپرم پوشش‌دار شده قوچ تالشی در حضور و عدم حضور زرده تخم مرغ

In order to study the effect of the presence of egg yolk and adding seminal plasma to coated sperm, the semen were collected from 4 taleshi rams by using artificial vagina. to coat the sperm, semen was collected within the tube containing tris-fructose-15% egg yolk. after the initial evaluation, the samples are mixed and centrifuged. after removing supernatant and diluting, samples were divided into 3 equal parts; they were cooled to 4°c and incubated for 44 hours. three methods (r, m and n) were performed to add seminal plasma. in r method, sample was centrifuged and supernatant were removed. in m method, sample was centrifuged and mixed with the supernatant. in n method, nothing was done and sample was kept at 4°c. sample, which obtained from each method, was split into three equal parts after that the amount of zero, 10 and 20% seminal plasma were added and aliquots incubated at 4°c. sperm motility, functional membrane integrity, viability and acrosome integrity were estimated after 4 hours. the lowest of sperm motility (10.83%) and viability (57.58%) were observed by r method (p < 0.05). the functional membrane integrity was higher in n method (61.33%) than r method (52%; p < 0.05) and there was no difference between r method or n method and m method. the methods did not effect on the acrosome integrity. therefore, removing egg yolk was not useful for coated ram spermatozoa after storage at 4°c.