study on antibiotic resistance pathern of e. coli in diarrheic calves of tabriz dairy farms

Introduction: diarrhea is a very important disorder in calves, especially in the first days after birth, due to mortality and economic losses. among infectious agents, pathogenic strains of escherichia coli (e. coli) have been known as the most important causes of diarrhea in calves. therefore, phenotypic and genotypic studies on e. coli strains that cause diarrhea in calves can be helpful for understanding their prevelance control methods.material and method: in this study, rectal swabs were collected from 1 to 30-day-old calves with diarrhea during 2021. the antibiotic-resistant pattern of the isolates was determined by the kirby-bauer disk diffusion susceptibility test. the prevalence of the pathogenic genes (bundle-forming pilus (bfp) and enteropathogenic attachment epithelial cells (eae) for enteropathogenic e. coli (epec) strains and verotoxigenic e. coli (vtec), shiga toxin (stx1 and stx2) strains was investigated using pcr.results and discussion: out of 105 samples, 81 isolates (85.05%) of e. coli were identified. the resistance of the isolates was observed for penicillin (56.84%), tetracycline (38.3%), sulfamethoxazole-trimethoprim (38.3%), gentamicin (29.6%), ceftazidime (29.2%), colistin (25.9%), imipenem (23.5%), ceftriaxone (22.21%), nalidixic acid (22.2%), ciprofloxacin (14.8%) and ofloxacin (9.9%) were detected in the isolates. furtheremore, 16% of the isolates had the eae gene and (24.7%) had both the stx1 and stx2 genes. in the present study, the infection rate of calves with diarrhea with vtec strains was higher than epec strains. according to the results of the present study, the presence of antibiotic resistance in e. coli isolates indicates the high importance of this bacterium in terms of epidemiology and public health.

study on antibiotic resistance pathern of e. coli in diarrheic calves of tabriz dairy farms

introduction: diarrhea is a very important disorder in calves, especially in the first days after birth, due to mortality and economic losses. among infectious agents, pathogenic strains of escherichia coli (e. coli) have been known as the most important causes of diarrhea in calves. therefore, phenotypic and genotypic studies on e. coli strains that cause diarrhea in calves can be helpful for understanding their prevelance control methods.material and method: in this study, rectal swabs were collected from 1 to 30-day-old calves with diarrhea during 2021. the antibiotic-resistant pattern of the isolates was determined by the kirby-bauer disk diffusion susceptibility test. the prevalence of the pathogenic genes (bundle-forming pilus (bfp) and enteropathogenic attachment epithelial cells (eae) for enteropathogenic e. coli (epec) strains and verotoxigenic e. coli (vtec), shiga toxin (stx1 and stx2) strains was investigated using pcr.results and discussion: out of 105 samples, 81 isolates (85.05%) of e. coli were identified. the resistance of the isolates was observed for penicillin (56.84%), tetracycline (38.3%), sulfamethoxazole-trimethoprim (38.3%), gentamicin (29.6%), ceftazidime (29.2%), colistin (25.9%), imipenem (23.5%), ceftriaxone (22.21%), nalidixic acid (22.2%), ciprofloxacin (14.8%) and ofloxacin (9.9%) were detected in the isolates. furtheremore, 16% of the isolates had the eae gene and (24.7%) had both the stx1 and stx2 genes. in the present study, the infection rate of calves with diarrhea with vtec strains was higher than epec strains. according to the results of the present study, the presence of antibiotic resistance in e. coli isolates indicates the high importance of this bacterium in terms of epidemiology and public health.