the effects of edta and propylene glycol on sperm quality and levels of extender elements during cryopreservation

The present study was designed to evaluate the effect of adding ethylene diamine tetra acetate (edta) and propylene glycol (pg) on extender calcium and magnesium and quality traits of frozen-thawed ghezel ram sperm. semen samples were collected from five rams (3-4 years) during the non-reproductive season once a week with 15 replicates. samples were diluted with tris-based extender without additive (control) and supplemented with 1.75 mm edta, 2% pg, and 7% pg (instead of glycerol). sperm quantitative characteristics were studied on 0, 20, 40, and 60 days of the storage after freeze-thawing. results indicated a negative correlation between the amount of calcium in seminal plasma and sperm parameters (p<0.01). also, there was a negative correlation between magnesium and sperm membrane integrity (p<0.01). furthermore, 1.75 mm edta and 2% pg significantly decreased calcium and magnesium levels in freeze-thawed samples compared to the control group (p<0.05). adding of 1.75 mm edta and 2% pg also significantly improved sperm quality parameters compared to the control group (p<0.05). it may be concluded that the addition of 1.75 mm edta and 2% pg to diluent significantly decreased the amount of calcium and magnesium of seminal plasma and improved the sperm parameters after the freeze-thawing process.

the effects of edta and propylene glycol on sperm quality and levels of extender elements during cryopreservation

the present study was designed to evaluate the effect of adding ethylene diamine tetra acetate (edta) and propylene glycol (pg) on extender calcium and magnesium and quality traits of frozen-thawed ghezel ram sperm. semen samples were collected from five rams (3-4 years) during the non-reproductive season once a week with 15 replicates. samples were diluted with tris-based extender without additive (control) and supplemented with 1.75 mm edta, 2% pg, and 7% pg (instead of glycerol). sperm quantitative characteristics were studied on 0, 20, 40, and 60 days of the storage after freeze-thawing. results indicated a negative correlation between the amount of calcium in seminal plasma and sperm parameters (p<0.01). also, there was a negative correlation between magnesium and sperm membrane integrity (p<0.01). furthermore, 1.75 mm edta and 2% pg significantly decreased calcium and magnesium levels in freeze-thawed samples compared to the control group (p<0.05). adding of 1.75 mm edta and 2% pg also significantly improved sperm quality parameters compared to the control group (p<0.05). it may be concluded that the addition of 1.75 mm edta and 2% pg to diluent significantly decreased the amount of calcium and magnesium of seminal plasma and improved the sperm parameters after the freeze-thawing process.