effects of nano-selenium and sodium selenite on serum selenoprotein p and gpx content in male broiler breeders

Introduction: fertility is necessary for hatchability of broiler breeder eggs. roosters as half part of the fertility have a great role and with increasing age fertility is declined. it has been revealed that phospholipid fraction of the avian spermatozoa membranes has high proportion of polyunsaturated fatty acids (pufa) and it is the reason why the spermatozoa are susceptible to free radical damages. to maintain sperm fertilizing ability, an antioxidant defense system is a crucial point. in avian semen the antioxidant system consists of natural antioxidants together with enzymes that have antioxidant characteristics such as glutathione peroxidase (gpx) and selenoprotein p (sepp1) protects sperm against free radicals and their destructive metabolites. antioxidants such as vitamin e and selenium (se) have remarkable roles in avian reproduction. to gain great reproductive performances in breeders, optimum level of antioxidant in diet is thought to be necessary. by using additives such as selenium (se) we can help delaying this reduction through antioxidant properties of se. replacing inorganic se by new source of se like the nano form in poultry diets can improve the fertility of broiler breeder eggs. this research was conducted to investigate the effect of nano-selenium (nano-se) in comparison with sodium selenite on serum selenoprotein p (sepp1) and glutathione peroxidase (gpx) content in broiler breeder roosters.material and method: a total of thirty arbor acres broiler breeder roosters (40 wks.) were randomly divided into five experimental groups. each of which included 3 replicates of 2 birds. according to the arbor acres broiler breeder manual, the amount of 160 grams of diet was allocated daily for each rooster which had 12% crude protein and 2800 kcal/kg metabolizable energy. after one-week adaptation, birds were fed the corn-soybean meal-based basal diet (t1) supplemented with 0.3 mg/kg sodium selenite (t2), 0.15 mg/kg nano-se (t3), 0.3 mg/kg nano-se (t4) and 0.6 mg/kg nano-se (t5). the duration of feeding experiment was four weeks. after the adaptation period, a 2.5 ml of blood sample was taken from each rooster. two weeks later, in the middle of the research, blood sampling was done again from each bird. four weeks after the treatment was done at the end of experiment, the roosters were humanely euthanized by cervical dislocation, the 3rd and last sampling was implemented at the end of experiment that as in the previous sampling was done, the blood samples were centrifuged and separated serum was stored in -20°c. then serum concentration of the antioxidant “sepp1” was measured by elisa method and “gpx” was analyzed using a spectrophotometry kit.results and discussion: by increasing the level of nanoselenium in diet, the serum concentration of sepp1 and gpx also increases (p < 0.05) and using 0.6 mg/kg nano-se in the diet reached the highest value. based on a consideration of all experiment indexes, in this research, 0.6 mg/kg is suggested to be the best level of supplementation of nano-se, and nano-se showed higher contents of serum sepp1 and gpx at the same amounts of nano-se and sodium selenite supplementation. in conclusion, dietary supplementation of nano-se was more effective than sodium selenite on serum sepp1 and gpx concentration of tested selenoproteins in broiler breeder males.

effects of nano-selenium and sodium selenite on serum selenoprotein p and gpx content in male broiler breeders

introduction: fertility is necessary for hatchability of broiler breeder eggs. roosters as half part of the fertility have a great role and with increasing age fertility is declined. it has been revealed that phospholipid fraction of the avian spermatozoa membranes has high proportion of polyunsaturated fatty acids (pufa) and it is the reason why the spermatozoa are susceptible to free radical damages. to maintain sperm fertilizing ability, an antioxidant defense system is a crucial point. in avian semen the antioxidant system consists of natural antioxidants together with enzymes that have antioxidant characteristics such as glutathione peroxidase (gpx) and selenoprotein p (sepp1) protects sperm against free radicals and their destructive metabolites. antioxidants such as vitamin e and selenium (se) have remarkable roles in avian reproduction. to gain great reproductive performances in breeders, optimum level of antioxidant in diet is thought to be necessary. by using additives such as selenium (se) we can help delaying this reduction through antioxidant properties of se. replacing inorganic se by new source of se like the nano form in poultry diets can improve the fertility of broiler breeder eggs. this research was conducted to investigate the effect of nano-selenium (nano-se) in comparison with sodium selenite on serum selenoprotein p (sepp1) and glutathione peroxidase (gpx) content in broiler breeder roosters.material and method: a total of thirty arbor acres broiler breeder roosters (40 wks.) were randomly divided into five experimental groups. each of which included 3 replicates of 2 birds. according to the arbor acres broiler breeder manual, the amount of 160 grams of diet was allocated daily for each rooster which had 12% crude protein and 2800 kcal/kg metabolizable energy. after one-week adaptation, birds were fed the corn-soybean meal-based basal diet (t1) supplemented with 0.3 mg/kg sodium selenite (t2), 0.15 mg/kg nano-se (t3), 0.3 mg/kg nano-se (t4) and 0.6 mg/kg nano-se (t5). the duration of feeding experiment was four weeks. after the adaptation period, a 2.5 ml of blood sample was taken from each rooster. two weeks later, in the middle of the research, blood sampling was done again from each bird. four weeks after the treatment was done at the end of experiment, the roosters were humanely euthanized by cervical dislocation, the 3rd and last sampling was implemented at the end of experiment that as in the previous sampling was done, the blood samples were centrifuged and separated serum was stored in -20°c. then serum concentration of the antioxidant “sepp1” was measured by elisa method and “gpx” was analyzed using a spectrophotometry kit.results and discussion: by increasing the level of nanoselenium in diet, the serum concentration of sepp1 and gpx also increases (p < 0.05) and using 0.6 mg/kg nano-se in the diet reached the highest value. based on a consideration of all experiment indexes, in this research, 0.6 mg/kg is suggested to be the best level of supplementation of nano-se, and nano-se showed higher contents of serum sepp1 and gpx at the same amounts of nano-se and sodium selenite supplementation. in conclusion, dietary supplementation of nano-se was more effective than sodium selenite on serum sepp1 and gpx concentration of tested selenoproteins in broiler breeder males.