بیان هترولوگ سم شبه شیگا نوع 2 در شرایط میکروگراویتی

ﺟﺎذﺑﻪ ﻧﻴﺮویی ﺗﺄﺛﻴﺮﮔﺬار ﺑﺮ ﺟﺎﻧﺪاران ﺳﺎﻛﻦ ﻛﺮه زﻣﻴﻦ از ﺟﻤﻠﻪ ﻣﻴﻜﺮوارﮔﺎﻧﻴﺴﻢﻫﺎﺳﺖ. ﺑﺎﻛﺘﺮی e. coli o157:h7 ﻋﻮارﺿﻲ ﻣﺎﻧﻨﺪ اﺳﻬﺎل ﺧﻮنی و ﺳﻨﺪرم اورﻣﻲ ﻫﻤﻮﻟﻴﺘﻴﻚ را در اﻧﺴﺎن اﻳﺠﺎد ﻧﻤﻮده و اﻳﻤﻦ ﺳﺎزی اﺻﻠﻲﺗﺮﻳﻦ راه ﻣﺒﺎرزه ﺑﺎ آن اﺳﺖ. زﻳﺮ واﺣﺪ b ﺳﻢ ﺷﺒﻪ ﺷﻴﮕﺎ ﻧﻮع 2 )(stx2b ﻛﻪ ﻋﺎﻣﻞ اﺗﺼﺎل ﺳﻢ ﺑﺎﻛﺘﺮی ﺑﻪ ﺳﻠﻮلﻫﺎی ﻫﺪف اﺳﺖ، از ﻛﺎﻧﺪﻳﺪﻫﺎی واﻛﺴﻦ ﻧﻮﺗﺮﻛﻴﺐ ﭘﻴﺸﮕﻴﺮی از ﺑﻴﻤﺎری اﺳﺖ. ﻧﻈﺮ ﺑﻪ اﻓﺰاﻳﺶ کمی و کیفی ﺑﻴﺎن ﺑﺮﺧﻲ ﭘﺮوﺗﺌﻴﻦﻫﺎی ﻧﻮﺗﺮﻛﻴﺐ در ﺷﺮاﻳﻂ بی وزنی ، ﻣﻴﺰان ﺑﻴﺎن ﭘﺮوﺗﺌﻴﻦ ﻧﻮﺗﺮﻛﻴﺐ stx2b در ﺷﺮاﻳﻂ بی وزنی ﺷﺒﻴﻪﺳﺎزی ﺷﺪه ﺑﺮ روی دﺳﺘﮕﺎه ﻛﻠﻴﻨﻮاﺳﺘﺖ ﻣﻮرد ﺑﺮرسی ﻗﺮار ﮔﺮﻓﺖ. ﭘﺲ از ﺗﺄﺋﻴﺪ ﭘﺮوﺗﺌﻴﻦ ﻧﻮﺗﺮﻛﻴﺐ ﺑﺎ روشﻫﺎی اﻳﻤﻨﻮﻟﻮژﻳﻚ، ﺑﻴﺎن آن در ﺷﺮاﻳﻂ ﺑﻲوزنی اﻧﺠﺎم ﺷﺪ و ﭘﺲ از ﺗﺨﻠﻴﺺ ﭘﺮوﺗﺌﻴﻦ ﺗﻮﺳﻂ ﺳﺘﻮن ﻛﺮوﻣﺎﺗﻮﮔﺮافی ، ﻣﻘﺪار آن ﻣﻮرد ﺳﻨﺠﺶ ﻗﺮار ﮔﺮﻓﺖ. ﻧﺘﺎﻳﺞ ﻳﺎﻓﺖ ﺷﺪه ﻧﺸﺎن دادﻧﺪ ﻛﻪ ﺑﻪرﻏﻢ ﻛﺎﻫﺶ ﻣﻴﺰان ﺑﻴﺎن در ﺷﺮاﻳﻂ بی وزنی ﻛﻪ اﺣﺘﻤﺎﻻً ﺑﻪدﻟﻴﻞ ﻋﺪم ﻫﻮادﻫﻲ ﻣﻨﺎﺳﺐ ﻣﺤﻴﻂ ﻛﺸﺖ ﺑﻮده اﺳﺖ، ﻫﻢ ﭼﻨﺎن ﭘﺮوﺗﺌﻴﻦ ﻧﻮﺗﺮﻛﻴﺐ در ﺷﺮاﻳﻂ بی وزنی ﺗﻮﻟﻴﺪ ﺷﺪ.

heterologous expression of shiga-like toxin type 2 in microgravity condition

gravity is an influential force on living organisms on earth, including microorganisms. e. coli o157: h7 causes complications such as bloody diarrhea and hemolytic uremic syndrome in humans and immunization is the main way to fight it. subunit b of shiga-like toxin type 2 (stx2b), binding agent of bacterial toxin to target cells, is a candidate for the recombinant vaccine to prevent disease. due to the quantitative and qualitative increase in the expression of some recombinant proteins under microgravity, the stx2b recombinant protein expression was examined under simulated microgravity conditions on clinostat device. after confirmation of the recombinant by immunological methods, its expression was performed under microgravity and after the protein purification by chromatographic column, its amount was measured. the results showed that despite the decrease in expression under microgravity, which was probably due to lack of proper aeration of the culture medium, recombinant protein was still produced under microgravity condition.