detection of human papilloma virus (hpv) infection in colorectal cancer and viral genome integration status

Background: colorectal cancer (crc) is the third common cause of malignancy in iran, and its incidence rate has been raised due to an increase in individual and environmental risk factors. objectives: the present study aimed to define the role of human papillomavirus (hpv) infection in crc, then by drawing a phylogenetic tree, we aimed to analysis the sequences of isolated hpvs, and finally determine the viral genome physical status for hpv-16. methods: a nested-pcr (npcr), direct sequencing, and inno-lipa hpv genotyping assay were carried out for hpv detection, and a quantitative real-time pcr assay (qrt-pcr) was conducted for the physical status of the viral genome. results: among 157 subsets, there were 66 cases and 91 controls. moreover, 5.7% (9/157) were hpv positive by npcr, among whom 9.1% (6/66) were cases and 3.3% (3/91) were control groups (p = 0.750). the qrt-pcr analysis showed three forms of integrated, episomal, and mixed forms in our three hpv-16 strains. a higher rate of hpv infection was detected in the crc cases; however, it was not statistically significant. conclusions: although there were limited positive results, higher ages (> 65) were related to hpv involvement and crc outcome. furthermore, 9.1% of the crc cases were infected with hpvs. the hpv-16 genome integration is observed in high-grade cancer cases with severe malignancy in crc patients.