hepatoprotective effect of allium hooshidaryae hydroalcoholic extract on acetaminophen-induced hepatotoxicity in male rats

Background]acetaminophen (apap) hepatotoxicity is a highly prevalent drug used for the treatment of pain. allium hooshidaryae is widely used as herbal medicine in western iran.[objectives]in this study, we investigated the protective effects of a. hooshidaryae methanolic extract (ahme) on liver damage induced by apap.[methods]rats were divided into six groups. groups 1 (negative control) and 2 (positive control) received normal saline; groups 3 received 200 mg/kg of ahme, and groups 4 6 received ahme at the doses of 50, 100, and 200 mg/kg once daily, by gavage, respectively. after the 14 days, except for groups 1 and 3, the other groups received 2 g/kg of apap. after 48 hours, hepatic tissue and blood sample were taken for histopathology and biochemical experiments.[results]apap induced a notable increase in serum levels of aspartate transaminase (ast, p lt; 0.001), alanine transaminase (alt, p lt; 0.001), alkaline phosphatase (alp, p lt; 0.001), lactate dehydrogenase (ldh, p lt; 0.01), nitric oxide (no, p lt; 0.001), and lipid peroxidation (lpo, p lt; 0.001) levels in hepatic tissue. it also significantly reduced the level of total antioxidant capacity (tac), total thiol groups (ttgs) as well as glutathione contents (gsh) in hepatic tissue (for all p lt; 0.001). following the use of ahme, a noteworthy improvement was observed in hepatic serum enzymes and oxidant/antioxidant parameters in liver tissue.[conclusions]this study demonstrated that ahme prevents apapinduced hepatotoxicity through improvement in the hepatic antioxidant status.