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A Rapid Modified Antibody-Capture Enzyme-Linked Immunosorbent Assay (ELISA) for Detection HSV-1 Primary Infection in Human
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نویسنده
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Ravanshad M ,Roustai MH ,Mostafaee A ,Sabahi F ,Soleimanjahi H ,Ziyaeyan M
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منبع
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shiraz e-medical journal - 2006 - دوره : 7 - شماره : 2 - صفحه:1 -5
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چکیده
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An antibody-capture enzyme-linked immunosorbent assay (elisa) based on the reaction between anti human igm and serum igm and hsv-1 conjugated with horseradish peroxidase (hrp) was developed. the specificity and sensitivity of the developed hsv-1 igm elisa were evaluated and compared with a commercially available test kit by using sets of well defined sera of known hsv-1 primary infection. for validation, 526 serum samples have been tested. the hsv-1 igm elisa had acceptable sensitivity and specificity, which were comparable with that of the commercial test kit. both assays showed some cross-reactivity with sera containing anti-hsv-2 antibodies.
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کلیدواژه
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Herpes Simplex Virus ,ELISA ,IgM
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آدرس
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tarbiat modares university, Faculty of Medical Sciences, Department of Virology, ایران, tarbiat modares university, Faculty of Medicine, Department of Medical Virology, ایران, razi university, Faculty of Medicine, Department of Immunology, ایران, tarbiat modares university, Faculty of Medicine, Department of Medical Virology, ایران, tarbiat modares university, Faculty of Medicine, Department of Medical Virology, ایران, tarbiat modares university, Faculty of Medicine, Department of Medical Virology, ایران
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پست الکترونیکی
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ravanshad@modares.ac.ir
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Authors
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